In these plants, the clones produced by a twig are similar in their genotype as well as in phenotype . Scientists have been much curious to apply this characteristics of plants on animals also to conserve the desired genotypes of some rare animals by making their clones. In higher animal, showing sexual reproduction, a zygote is formed after fertilization of the egg by spermatozoa. It was revealed by the scientists through several experiments that only the egg and/or zygote has the potential to produce a whole individual from a single cell. Gurdon of Oxford University applied this fact while performing an experiment on frog. He destroyed the nucleus of an unfertilized egg of frog by treating with U.V rays and transferred the nucleus of intestinal epithelial cell of tadpole into the egg cell .

Algae and cyanobacteria as a source of novel bioactive compounds for biomedical applications. Advances in Cyanobacterial Biology, Elsevier Publication, Chapter 12, 2020. Divyashree M, Deekshit V K, Krishna Kumar Ballamoole, Veena Shetty A, Anirban Chakraborty, Indrani Karunasagar. Occurrence of antibiotic resistance among Gram negative bacteria isolated from effluents of fish processing plants in and around Mangalore . Establishment of three-dimensional cell culture to generate biomimetic cancer models. Pinto, D. S., Prithvisagar, K. S., Rohit, A., Karunasagar, I., Karunasagar, I., & Kumar, B. K.

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Bioprospecting of eco friendly natural dyes using novel marine bacteria as an efficient…. Bioprospecting of antimicrobial Carica papaya leaf extract for finishing of fabrics in health care industries. With more than 15 years of experience in research and teaching, she has attended several conferences and been resource person at National and International level. Her latest visit to the U.S.A where she was invited as speaker to present a research paper on Zika virus at the First International Conference on Zika Virus 2017 held at Washington D.C, 2017.

A molecule’s properties determines, how rapidly an electric field can move the molecule through a gelatinous medium. Extraction– DNA extracted from the cell by cell lysis . If the content of DNA is limited then DNA can be amplified by Polymerase chain reaction . It is technique to identify a person on the basis of his/her DNA specificity. Mention the contribution of genetic maps in human genome project.

You can find more details by visiting the additional pages to view historical data, charts, latest news, analysis or visit the forum to view opinions on the VNTR quote. A) Explain DNA polymorphism as the basis of genetic mapping of human genome. Expand VNTR and describe its role in DNA finger printing. List any two applications of DNA finger printing technique. It is used in forensic science to identify potential crime suspects. Sustainable utility of Carica papaya leaf extract for the production of ecosafe medical textiles.

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Presently, he is engaged in the following areas-a) Development of an in vivo Drosophila model to study toxicant/radiation induced hematopoietic perturbations and b) Host-Microbe interaction and environmental toxicants. In such condition, if the cells from the patient himself are taken and cultured to form desired organ. The organs developed in such manner will be easily acceptable by the patient, and there will be no possibility of its rejection as often occurs otherwise. Dr. Sudarshan Kini is a Bionanotechnologist who obtained Ph.D. from the Indian Institute of Technology Bombay and has Post-Doctoral experience from Manipal University. His research areas are the fabrication of biomaterial scaffolds for the 3D culturing of cancer cells and Pseudomonas biofilms.

Melanogaster, a non-target organism, at sub-organismal as well as organismal level. Further, his studies also highlight the potential of D. Melanogaster as an alternative animal model for in-vivo assessment of environmental chemical mediated toxicity, which might have relevance to higher organisms. Later, he joined CNRS-Centre de Biologie du Développement, Toulouse, France as a postdoctoral researcher and worked on deciphering the function of the Drosophila hematopoietic niche to maintain blood cell homeostasis.

Lomous Kumar

According to regulations, Indian residents are eligible to trade all available products on domestic Indian markets, including futures and options. On non-Indian markets, Indian residents are prohibited from trading futures, options or margin-based products but are permitted to trade stocks, bonds and ETFs. Take your analysis to the next level with our full suite of features, known and used by millions throughout the trading world. World-class wealth management using science, data and technology, leveraged by our experience, and human touch. The price-to-book ratio is a company’s current market price to its Book Value. Traditionally, any value under 1.0 is considered a good P/B value, indicating a potentially undervalued stock.

The DNA fingerprint of the person matching the one obtained from sample collected from scene of crime can give a clue to the actual criminal. The term electrophoresis describes the migration of charged particles under the influence of an electric field. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provides the driving force.

These fragments are prepared for commercial and hybridization technology either for finding complementary sequence or in the diagnosis of some diseases. Industrial BiotechnologyApplication of bacterial biopigments as alternative dyes in the textile industry. Divyashree M, Madhu K Mani, Mamatha B S, Sudarshan Kini.

In this experiment a few of the many transplanted eggs could develop into tadpoles. These developed tadpoles were identical in genotype and phenotype to their parents. This nuclear transplantation technique devised by Gurdon is still being used in cloning practice in some modified manner.

She has secured second rank in MSc Microbiology from University of Mysore. Pathogenesis and membrane-based antibiotic resistance mechanisms of bacteria. Cloning is the process of producing many identical organisms , generally used to produce new plants with similar characteristics. In higher animals, clones are produced by nuclear transplantation technique in which the nucleus from a somatic cell is transferred into an unfertilized enucleated egg. The world’s most famous sheep ‘Dolly’ was a clone produced by this method. Many incurable diseases which are not curable so far, may be cured effectively in near future by applying the processes relating with cloning and genetic engineering.

A previous report suggested that the ORF 94 VNTR is highly unstable, varying in the number of tandem repeats during single passages from shrimp to other crustaceans. High titre stocks of each virus were produced by injection in specific pathogen-free Pacific white shrimp Litopenaeus vannamei with filtered infected tissue extracts, and the genotypes were confirmed. Each stock was then used to feed SPF Pacific white shrimp, freshwater crayfish (Cherax sp.) and freshwater prawns Macrobrachium rosenbergii through 3 successive passages involving alternative hosts at each level.

Dr. Krishna Kumar Ballamoole is a molecular microbiologist, obtained Ph.D. from Manipal University. His research focuses on understanding and characterizing the genomic patterns that drives the evolution and infection capabilities of microbial pathogens of humans and animals. In addition to pursuing the main research interest, the he is also involved in developing monoclonal antibody and nucleic acid based rapid diagnostics for the detection of pathogens. The white spot syndrome virus genome contains 3 variable number tandem repeat regions, located in open reading frame 75, ORF94 and ORF125, which have been employed for molecular epizootiological studies.

He is also working on developing quantum dot-based fluorescent nanobiosensors to detect MRSA (Methicillin-resistant Staphylococcus aureus). She has obtained her Ph.D. degree from Department of Microbiology, University of Mysore in the year 2012. Her research interests include virulence in bacterial pathogens, quorum sensing and isolation of anti-quorum sensing molecules from phytal sources and attenuation of quorum sensing in bacteria.

Genome https://1investing.in/ of clinical isolate of Campylobacter fetus subspecies fetus MMM01 from India reveals genetic determinants of pathogenesis and adaptation. Aditya, V., Kotian, A., Saikrishnan, S., Rohit, A., Divyashree, M., Karunasagar, I., Deekshit, V.K. Effect of ciprofloxacin and in-vitro gut conditions on biofilm of Escherichia coli isolated from clinical and environmental sources. Deekshit VK, Maiti B, Kumar BK, Kotian A, Pinto G, Reantaso M, Karunasagar I, Karunasagar I. Antimicrobial resistance in fish pathogens and alternative risk mitigation strategies. A plant biotechnologist by training, having 30 years of experience in academics and research. I have worked extensively on plant tissue culture, conservation, biodiversity and molecular markers in tree species and Pteridophytes. My team has screened several Pteridophytes for phytoremediation of heavy metals from contaminated soil and liquid systems.

Taqman real-time PCR was conducted on samples from each group to confirm infection and quantify viral genetic loads. ORF94 VNTR genotype analysis conducted on samples from each of the 43 passage groups indicated no variations in the VNTR number in either genotype TRS5 or genotype TRS7. This finding is contrary to the previous report and suggests that ORF94 VNTR are stable during multiple passages in these crustaceans. Dr. Akshath completed his Ph.D. at CSIR-CFTRI, Mysore, with a specialization in Biosensors and Bio-imaging. His work was focused on nanoparticle-based biosensors to detect food contaminants and toxins using enzymes/aptamers as biorecognition elements.

My research emphasizes on virulence in foodborne/zoonotic pathogens and the use of antisense technologies to combat drug resistance in bacteria. My current research focuses on developing diagnostic/ immunotherapeutic assays for animal viruses and isolation of bioactive compounds from marine sources. At present my research area and interest focusses on development of rapid molecular detection techniques, development of transgenic zebrafish lines for human diseases, Human /animal parasitology, and Molecular Immunology. Dr. Praveen Rai did PhD from Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar.

• Many incurable diseases which are not curable so far, may be cured effectively in near future by applying the processes relating with cloning and genetic engineering.
• Instead of ,nucleus from the donor cell which is in G0or G1, stage is substituted into enucleated egg cell .
• His work was focused on nanoparticle-based biosensors to detect food contaminants and toxins using enzymes/aptamers as biorecognition elements.
• These allow analyzer to identify a particular person DNA, the occurrence and frequency of a particular genetic pattern contained in this X-ray film.
• Melanogaster, a non-target organism, at sub-organismal as well as organismal level.

The credit risk definitions which are used in such cloning include blastomere separation, nuclear transplantation and the Honolulu technique. Animal cloning Embryonic cell in animals, are deprived of their totipotency by the time they enter into gastrula stage. So animal cloning is some what more difficult than plant cloning. On the basis of aims and end products animal cloning is of two types Reproductive and Therapeutic.